尿素包合法富集猪油中油酸的工艺优化及降血脂作用
Optimization of Enrichment of Oleic Acid in Lard by Urea Inclusion Method and Its Effect on Metabolism of High Fat Mice
王佳佳 1徐慧敏 1孟鑫1
作者信息
- 1. 锦州医科大学食品与健康学院(锦州 121000)
- 折叠
摘要
采用尿素包合法富集猪油中油酸,通过单因素试验分别考察包合时间、脲酯比、醇脲比对油酸得率的影响,并利用响应面法优化油酸富集提取工艺.当包合时间8 h、脲酯比1.7∶1、醇脲比4.27∶1时,油酸得率为53.28％.建立高脂血症小鼠模型,考察油酸对高脂血症小鼠血脂代谢的影响,与模型组相比,小鼠血清中TC、TG、LDL-C含量显著降低,HDL-C含量显著增加.肝脏组织切片与阳性药物对照组接近,减小肝脏的病变程度,证明摄入适量的油酸可有效降低高脂血症小鼠血脂水平.
Abstract
Oleic acid in lard was enriched by urea inclusion method.The effects of inclusion time,urea-ester ratio and alcohol-urea ratio on oleic acid yield were investigated by single factor test,and the extraction process was optimized by response surface method.When the inclusion time was 8 h,the ratio of urea to ester was 1.7:1,and the ratio of alcohol to urea was 4.27:1,the oleic acid yield was 53.28％.A hyperlipidemia mouse model was established to investigate the effect of oleic acid on lipid metabolism in hyperlipidemia mice.Compared with the model group,the contents of TC,TG and LDL-C in serum of mice were significantly decreased,and the contents of HDL-C were significantly increased.The liver tissue sections were similar to those of the positive drug control group,which reduced the degree of liver lesions,proving that the intake of appropriate oleic acid can effectively reduce the blood lipid level of hyperlipidemia mice.
关键词
猪油/油酸/降血脂Key words
lard/oleic acid/reduce blood lipid引用本文复制引用
基金项目
辽宁省自然科学基金资助(20180550487)
出版年
2024
NETL
NSTL
维普
万方数据