Optimization of Enrichment of Oleic Acid in Lard by Urea Inclusion Method and Its Effect on Metabolism of High Fat Mice
Oleic acid in lard was enriched by urea inclusion method.The effects of inclusion time,urea-ester ratio and alcohol-urea ratio on oleic acid yield were investigated by single factor test,and the extraction process was optimized by response surface method.When the inclusion time was 8 h,the ratio of urea to ester was 1.7:1,and the ratio of alcohol to urea was 4.27:1,the oleic acid yield was 53.28％.A hyperlipidemia mouse model was established to investigate the effect of oleic acid on lipid metabolism in hyperlipidemia mice.Compared with the model group,the contents of TC,TG and LDL-C in serum of mice were significantly decreased,and the contents of HDL-C were significantly increased.The liver tissue sections were similar to those of the positive drug control group,which reduced the degree of liver lesions,proving that the intake of appropriate oleic acid can effectively reduce the blood lipid level of hyperlipidemia mice.
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万方数据
维普
