Formulation Optimization and Hypoglycemic Activity of Almond and Pueraria thomsonii Benth Meal Replacement Biscuits
Taking sensory scores,hardness,DPPH free radical scavenging rate,ABTS+·radical scavenging rate,total antioxidant capacity,and α-glucosidase inhibitory activity as the inspection indicators,the effects of the amount of almond meal powder,whole kudzu powder and xylooligosaccharide on the quality of almond and Pueraria thomsonii Benth(PTB)meal replacement biscuits were observed.On the basis of a single factor test,the optimum formula of meal replacement biscuit was determined with the comprehensive score as response value.Drosophila type 2 diabetes model induced by high glucose was employed in vivo,and the hypoglycemic activity of meal replacement biscuits was evaluated by total protein content,triglyceride content and trehalose content.The results showed that the optimal formula of meal replacement biscuits was as following:almond meal powder 25%,kudzu powder 30%,xylo-oligosaccharide 2.5%,low-gluten flour 40%,corn starch 10%,water 48%,corn germ oil 14%,table salt 1.5%,double-effect baking powder 1.0%,and sodium hydrogen phosphate 0.7%.The scavenging ability against DPPH·free radicals,ABTS+·free radical of Almond and PTB meal replacement biscuits were 8.470±0.819 mg/mL and 5.235±0.265 mg/mL,respectively,while total antioxidant capacity was 0.184.Almond and PTB meal replacement biscuits had certain antioxidant activity while in vitro hypoglycemic experiment and Drosophila survival experiment showed that it had hypoglycemic effect.
almond meal powder-PTBmeal replacement biscuitoptimum formularesponse surface methodologydrosophila type 2 diabetes model