Intervention Effect of Deer Heart Peptide on Acute Alcoholic Liver and Kidney Injury in Mice
Explore the effect of deer heart peptide on alcoholic liver and kidney injury in mice.Twenty healthy male Kunming mice were randomly divided into blank group,control group,low dose and high dose groups.Except the blank group,the other 3 groups were administrated with 56-degree liquor to establish the alcoholic liver and kidney injury model.The body weight and organ wet weight of mice were weighed,the liver and kidney indexes were measured,the levels of oxidative stress were detected by detecting the contents of serum antioxidant indexes malondialdehyde(MDA)and superoxide dismutase(SOD),and the activity of acetaldehyde dehydrogenase(ALDH)was detected by enzyme label and kit.The mRNA expression levels of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor(TNF-α)were detected by qPCR af-ter the total RNA of liver and kidney was obtained by Trizol.The results showed as follows:Compared with the control group,the liver index of high and low dose of deer heart peptide group decreased significantly,the liver index of high and low dose of deer heart peptide group was significantly lower than that of the control group,and the liver index of low dose was significantly lower than that of high dose group.The results showed that the kidney index of the blank group was significantly different from that of the control group,while that of the control group was significantly different from that of the low-dose group and that of the high-dose group.The SOD content in the high and low dose groups was higher than that in the control group.Compared with the control group,MDA content in high and low dose groups was decreased.Compared with the control group,ALDH values in high and low dose groups were significantly increased.The expression levels of TNF-α,IL-1β and IL-6 in the high-low dose group were significantly decreased,compared with the control group.These results indicated that deer heart peptide could intervene and prevent alcoholic liver and kidney injury in mice.
deer heart peptideanti-oxidationliver and kidney injuryinflammatory factor
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NSTL
万方数据
维普
