苜蓿提取物不同极性部位抗氧化活性研究
Antioxidant Activities of the Different Polarity Parts of Extract from Medicago sativa L.
李倩 1孙勇康 1安琼 1王贞香 1张振 1梁艳婷1
作者信息
- 1. 河西学院医学院,甘肃省河西走廊特色资源利用重点实验室(张掖 734000)
- 折叠
摘要
对苜蓿地上部分不同极性部位成分进行鉴别,并比较不同极性部位的抗氧化活性.采用苜蓿的70％醇提物为样本,使用石油醚、乙酸乙酯和正丁醇对苜蓿醇提物进行萃取,得到不同极性部位并对其进行成分鉴别.采用硝酸铝显色法、福林酚显色法和苯酚-硫酸法测定黄酮、酚类和糖的含量.采用ABT、DPPH、OH-清除率和总还原能力比较各部位的抗氧化活性.黄酮主要分布在乙酸乙酯与石油醚部位,含量为0.144 6 mg/mL和0.086 9 mg/mL;酚类主要分布于乙酸乙酯和正丁醇部位,含量为2.697 5 mg/mL和2.574 9 mg/mL;糖主要分布在水和石油醚部位,含量为1.154 2 mg/mL和1.069 9 mg/mL.4个指标综合评价得出水部位抗氧化能力最强,正丁醇部位次之.紫花苜蓿水部位和正丁醇部位抗氧化能力最强,抗氧化的主要活性成分为糖.
Abstract
To explore the antioxidant activity of different polarity part of ethanol extract from Medicago sativa L.in vitro,taking the 70％ethanol extract of Medicago sativa L.as a sample,the different polar parts were obtained by extraction with petroleum ether,ethyl acetate and n-butanol,which were identified the main chemicals.Silver nitrate colorimetric method,Folin-Ciocalteu method and Phenol-sulfuric acid method were used to determine the content of flavonoids,polyphenols and glycosides.DPPH,ABTS,Hydroxyl and total reducing capacity were used to evaluate its anti-oxidant activity in vitro.Flavonoids were mainly distributed in ethyl acetate and petroleum ether,and the contents were 0.144 6 mg/mL and 0.086 9 mg/mL;Phenols were mainly distributed in ethyl acetate and n-butanol,and the contents were 2.697 5 mg/mL and 2.574 9 mg/mL;Glycosides were mainly distributed in water and petroleum ether,and the contents were 1.154 2 mg/mL and 1.069 9 mg/mL.The water part exhibited the best antioxidant capacity,then followed by n-butanol part.The water part and n-butanol part extract of Medicago sativa L.had the strongest antioxidant capacity.The active ingredients with an antioxidant effect were glycosides.
关键词
苜蓿/不同极性部位/成分鉴别/含量测定/抗氧化活性Key words
Medicago sativa L./different polar parts/identification of components/content determination/antioxidant activity引用本文复制引用
基金项目
甘肃省教育科技创新项目(2022B-172)
甘肃省教育科技创新项目(2022B-205)
甘肃省高等学校创新基金项目(2021A-120)
甘肃省大学生国家级创新训练项目(202310740002)
甘肃省大学生国家级创新训练项目(202210740004)
出版年
2024
NETL
NSTL
维普
万方数据