Uncertainty Evaluation for the Determination of Five Poppy Alkaloids in Seasoning Powder by HPLC-MS/MS Method
The contents of five poppy alkaloids in seasoning powder were determined by high performance liquid chromatog-raphy-tandem mass spectrometry,and the uncertainty of the method was evaluated.According to BJS201802 determination of morphine,codeine,papaverine,narcotin and tibaine in food,the mathematical model of morphine and codeine uncertainty was constructed,the whole process of measurement was analyzed,the source of uncertainty was analyzed,and the synthetic uncertainty and extended uncertainty were obtained.The result showed that the main sources of uncertainty in determining the content of five poppy alkaloids in seasoning powder were the preparation of standard solution and the fitting of standard curve;When the contents of morphine codeine,papaverine,narcotin and thebaine in flavoring powder were 76.87,76.35,15.77,15.78 and 15.71 μg/kg,their extended uncertainties were 8.96,9.72,1.69,2.27 and 2.49 μg/kg,respectively(k=2).It could provide reference for the detection of morphine,codeine,papaverine,narcotin and Tibaine in seasoning powder.
high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)seasoning powderpop-py alkaloiduncertainty
NETL
NSTL
万方数据
维普
