Determination of 6 Compounds of Vitamin B in Health Foods by HPLC
Establish a method of rapid determination of nicotinic acid,nicotinamide,thiamine hydrochloride,pyridoxine hydrochloride,pyridoxal hydrochloride and pyridoxamine dihydrochloride in health foods by ultrasonic extraction-liquid chromatography.Chromatographic condition is that SB C18 column(4.6 mm×250 mm,5 μm),mobile phase 0.1％trifluoroacetic acid(A)-methanol(B).The elution condition is that 0-4 min,3％B;4-9 min,15％B;9-10 min,15％*20％B;10-11 min,60％-95％B;11-15 min,95％B;15-15.5 min,95％*3％B;15.5-18 min,3％B.Detection wavelength is 280 nm.The results show that the linear range of nicotinic acid,nicotinamide,thiamine hydrochloride,pyridoxine hydrochloride,pyridoxal hydrochloride and pyridoxamine dihydrochloride are 0.5-50 μg/mL,and the correlation coefficient is 0.999 9.The recoveries at 0.125,0.500 and 1.250 mg/kg are between 90％and 102％,with the relative standard deviation ≤4％.This method is sample,fast,with short detection period,and can provide simultaneous determination of nicotinic acid,nicotinamide,thiamine hydrochloride,pyridoxine hydrochloride,pyridoxal hydrochloride and pyridoxamine dihydrochloride.
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