摘要
建立超声萃取-液相色谱联用法快速测定保健食品中烟酸、烟酰胺、盐酸硫胺素、盐酸吡哆醇、盐酸吡哆醛和盐酸吡哆胺的检验方法.色谱条件:SB C18柱(4.6 mm × 250 mm,5 μm),流动相A为0.1％三氟乙酸、流动相B为甲醇.洗脱程序:0～4min,3％B;4～9 min,15％B;9～10 min,15％～20％B;10～11 min,60％～95％B;11～15 min,95％B;15～15.5 min,95％～3％B;15.5～18 min,3％B.检测波长280 nm.结果表明,烟酸、烟酰胺、盐酸硫胺素、盐酸吡哆醇、盐酸吡哆醛和盐酸吡哆胺线性范围为0.5～50 μg/mL,相关系数0.999 9.以0.125,0.500和1.250 mg/kg为3个水平加标,测得的回收率在90％～102％,SRSD≤4％.该方法简单、快捷、检测时间短,可同时对保健食品中的烟酸、烟酰胺、盐酸硫胺素、盐酸吡哆醇、盐酸吡哆醛和盐酸吡哆胺进行检测.
Abstract
Establish a method of rapid determination of nicotinic acid,nicotinamide,thiamine hydrochloride,pyridoxine hydrochloride,pyridoxal hydrochloride and pyridoxamine dihydrochloride in health foods by ultrasonic extraction-liquid chromatography.Chromatographic condition is that SB C18 column(4.6 mm×250 mm,5 μm),mobile phase 0.1％trifluoroacetic acid(A)-methanol(B).The elution condition is that 0-4 min,3％B;4-9 min,15％B;9-10 min,15％*20％B;10-11 min,60％-95％B;11-15 min,95％B;15-15.5 min,95％*3％B;15.5-18 min,3％B.Detection wavelength is 280 nm.The results show that the linear range of nicotinic acid,nicotinamide,thiamine hydrochloride,pyridoxine hydrochloride,pyridoxal hydrochloride and pyridoxamine dihydrochloride are 0.5-50 μg/mL,and the correlation coefficient is 0.999 9.The recoveries at 0.125,0.500 and 1.250 mg/kg are between 90％and 102％,with the relative standard deviation ≤4％.This method is sample,fast,with short detection period,and can provide simultaneous determination of nicotinic acid,nicotinamide,thiamine hydrochloride,pyridoxine hydrochloride,pyridoxal hydrochloride and pyridoxamine dihydrochloride.
基金项目
贵州省教育厅贵州省高等学校特色重点实验室项目(黔教合KY[2020]013)
NETL
NSTL
维普
万方数据