HPLC-MS/MS法测定猪肉中克伦特罗、莱克多巴胺及沙丁胺醇
Determination of Clenbuterol,Ractopamine and Salbutamol in Pork by HPLC-MS/MS
双杨 1赵燕 2夏林林 1吴磊磊 1陈银华1
作者信息
- 1. 十堰市食品药品检验检测所(十堰 442000)
- 2. 十堰市黄龙镇中心小学(十堰 442000)
- 折叠
摘要
采用高效液相色谱-串联质谱法测定猪肉中克伦特罗、莱克多巴胺及沙丁胺醇,内标法定量.结果显示,在1.0～50.0 ng/mL浓度范围内3种化合物线性关系良好,线性相关系数均大于0.999,方法检出限为0.2 μg/kg时,3种化合物响应值均大于3,方法定量限为0.5 μg/kg时,3种化合物响应值均大于10.在1.0,4.0和8.0 μg/kg浓度水平上加标回收率为81.0％～94.0％,相对标准偏差(n=7)分别为1.83％,3.07％和3.50％,满足方法验证要求.
Abstract
Determine the clenbuterol,ractopamine and salbutamol in pork by high performance liquid chromatography-tandem mass spectrometry.The results showed that the linear relationship among the three compounds was good in the concentration range of 1.0-50.0 ng/mL;The linear correlation coefficients were greater than 0.999;When the detection limit of the method was 0.2 μg/kg,the response values of all three compounds were greater than 3;When the limit of quantification of the method was 0.5 μg/kg,the response values of all three compounds were greater than 10.The recoveries were 81.0％-94.0％at the concentrations of 1.0,4.0 and 8.0 μg/kg,and the relative standard deviations were 1.83％,3.07％and 3.50％.The results meet the requirement of method verification.
关键词
高效液相色谱-串联质谱法/克伦特罗/莱克多巴胺/沙丁胺醇Key words
high performance liquid chromatography-tandem mass spectrometry/clenbuterol/ractopamine/salbutamol引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据