Determination of Residues of Bentazone and Its Metabolites in Tea by HPLC-MS/MS
A QuEChERS-liquid chromatography-mass spectrometry(LC-MS/MS)method was established a method for the determination of bentazone and its metabolites(6-hydroxymetazone and 8-hydroxymetazone residues)in tea.The tea samples were soaked in water for a period of time,extracted with 1％acetonitrile acetate solution as following,then purified by the mixed purification PSA,C18,GCB with anhydrous magnesium sulfate,and detected by liquid chromatography-mass spectrometry(LC-MS/MS)in multi-reaction monitoring(MRM)mode for the residues of methomyl and its metabolites named 6-hydroxymetazone and 8-hydroxymetazone in tea,as well as quantified by external standard method.Good linearity(r＞0.99)was observed in the range of 1-100 μg/L for mace and its metabolites under the optimized condition,and the average recoveries of the samples were 83.4％-99.2％with the relative standard deviation(n=6).f 1.2％-7.3％,the limits of detection of the method were 0.014-0.42 μg/kg and the limits of quantification were 10 μg/kg at three spiked levels(10,20 and 100 μg/kg).It's not hard to make the conclusion that the method is suitable for the simultaneous determination of bentazone and its metabolites in tea.
liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)QuEChERStearesidues
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万方数据
维普
