首页|超声前处理对牡蛎蛋白水解度的影响

超声前处理对牡蛎蛋白水解度的影响

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为提高牡蛎蛋白酶解液的水解度,利用超声对牡蛎进行预处理,用中性蛋白酶进行水解.在单因素基础上,利用响应面法对超声前处理的超声功率、超声时间、超声温度、料液质量比工艺参数进行优化.试验表明,经超声处理后,在相同酶解条件下,牡蛎蛋白水解度得到有效提高.结果显示,超声前处理最佳工艺参数为超声功率370 W、超声时间35 min、超声温度55 ℃、料液质量比1∶8,通过中性蛋白酶进行酶解,水解度达32.15%,比未经过超声预处理的牡蛎溶液水解度提高12.02%.
The Effect of Ultrasound Pretreatment on the Hydrolysis Degree of Oyster Protein
To improve the hydrolysis degree of oyster protease hydrolysate,ultrasound was used for pretreatment of oysters,and then neutral protease was used for hydrolysis.On the basis of single factor analysis,the response surface methodology was used to optimize the process parameters of ultrasonic treatment,including ultrasonic power,ultrasonic time,ultrasonic temperature,and material liquid mass ratio.It was found that under the same enzymatic hydrolysis condition,the hydrolysis degree of oyster protein was effectively improved after ultrasonic treatment.The results showed that the optimal process parameters for pre-treatment before ultrasound were ultrasound power of 370 W,ultrasound time of 35 min,ultrasound temperature of 55 ℃,material to liquid mass ratio of 1∶8,and enzymatic hydrolysis using neutral protease.The hydrolysis degree reached 32.15%,which was 12.02%higher than that of the untreated oyster solution.

oystersultrasound pre-treatmentenzymatic hydrolysishydrolysis degree

罗联钰、徐清清、朱金燕、魏维鑫、吴清朋、刘家光

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福建正味生物科技有限公司(福州 350015)

牡蛎 超声前处理 酶解 水解度

福州市科技计划项目福州市科技重大项目

2020-N-182022-ZD-025

2024

食品工业
上海市食品工业研究所

食品工业

影响因子:0.47
ISSN:1004-471X
年,卷(期):2024.45(4)
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