In order to study effects and optimization fermentation technology of liduid fermentation of Hirsutella sinensis on highland barley,it has been fermented by Hirsutella sinensis,and mycelium biomass and protein content have been determined as index.Highland barley has been fermented by Hirsutella sinensis,and time and temperature of fermentation,inoculation amount,rotation speed and liquid volume are optimized by single factor test and response surface test.The optimal fermentation technology has been selected as follows:fermentation time is 6 d under 22 ℃,inoculation amount is 3％,rotation speed is 135 r/min,and liquid loading volume is 40％.Under the optimal condition,mycelial biomass is 0.017 8±0.000 8 g/mL and protein content is 2.02％±0.16％in fermentation broth.The mycelial biomass and protein content are 1.63 times and 2.15 times higher than those before fermentation(P＜0.05).Highland barley can be used as culture medium for liquid fermentation of Hirsutella sinensis,and the biomass and protein content of mycelium have been increased after fermentation,which results from transformation of nutrients of highland barley.The research results provide a new way for the research of biological transformation of highland barley.
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