Optimization of Ultrasound-Assisted Extraction of Polysaccharides from Polygonum perfoliatum L.by Central Composite Design-Response Surface Methodology
To investigate the optimal process for the ultrasonic-assisted extraction of polysaccharides from Polygonum perfoliatum L.,using a single-factor experimental design as the basis,a three-factor five-level central composite design(CCD)-response surface methodology(RSM)method was employed to study the effects of ultrasonic time,liquid-to-material ratio and extraction temperature on the extraction yield of polysaccharides.The optimal process for ultrasonic-assisted extraction of polysaccharides was determined based on the results.The study revealed that the optimal process for ultrasonic-assisted extraction of polysaccharides involved an ultrasonic time of 30 min,a liquid-to-material ratio value of 37 mL/g,2 cycles of ultrasonic extraction at 65 ℃,resulting in a polysaccharide extraction yield of 5.38％(SRSD=0.53％).The theoretical prediction values closely matched the experimental results.The optimized extraction process exhibited high extraction yield,stability,and feasibility.
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