采用膜滤方法从紫薯淀粉加工副产物中制备花色苷,采用单因素试验探讨柠檬酸浓度、提取时间、料液比对提取效果的影响,确定紫薯花色苷较适宜的提取条件,即柠檬酸浓度2％,提取时间60 min,料液比1∶6(g/mL).通过小鼠饲喂试验,测定血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)等指标以探究紫薯花色苷(purple potato anthocyanins,PSPAs)对高脂膳食小鼠血脂的影响.结果表明,PSP As能够显著降低高脂膳食小鼠血清中TC、TG、LDL-C含量及升高HDL-C含量,且呈现一定的量效关系.与模型对照组相比,低剂量组(13 mg/kg)的综合降血脂效果最好,能使小鼠血清TC、TG、LDL-C含量分别下降15.10％,21.77％和33.33％,HDL-C升高6.29％,且低剂量的PSPAs能使高脂膳食小鼠血清TG、HDL-C含量恢复到正常水平,效果与阳性对照组无差异.
The Hypolipidemic Activity of Anthocyanins Extracted from Byproducts Generated during the Starch Processing of Purple Potatoes
Abstrad Anthocyanins were prepared from purple sweet potato starch processing by-products by membrane filtration method.The effects of citric acid concentration,extraction time and solid-liquid ratio on the extraction effect were investigated by single factor experiment.The suitable extraction condition of purple sweet potato anthocyanins was determined as citric acid concentration of 2％,extraction time of 60 min,and solid-liquid ratio of 1∶6(g/mL).Through the mouse feeding experiment,total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)in serum were measured to investigate the effects of purple potato anthocyanins(PSPAs)on blood lipids in mice fed a high-fat diet.The results showed that PSPAs could significantly reduce the contents of TC,TG and LDL-C,and increase the content of HDL-C in the serum of high-fat diet mice,and showed a certain dose-effect relationship.Compared with the model control group,the comprehensive lipid-lowering effect of the low-dose group(13 mg/kg)was the best,which could reduce the serum TC,TG and LDL-C content of mice by 15.10％,21.77％and 33.33％,and increase HDL-C by 6.29％,respectively.The low-dose PSPAs could restore the serum TG and HDL-C content of high-fat diet mice to normal levels,and the effect was not different from that of the positive control group.
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