Process Optimization and Antioxidant Activity Study of Polysaccharide from Dendrobium officinale by Ultrasonic and Enzyme-Assisted Extraction
Using Dendrobium officinale as raw material,the suitable extraction condition and the antioxidant activity of Dendrobium officinale polysaccharides(DOP)were studied.The single factor experiments and orthogonal experiments were used to investigate the effects of the six factors on the extraction of polysaccharides,including enzyme addition,enzymolysis temperature,enzymolysis time,enzymolysis pH,ultrasonic power and ultrasonic time.The antioxidant activity of DOP was investigated by DPPH·,OH·and O2-·scavenging effects,and ferric iron reduction ability.The results demonstrated that the ultrasonic extraction optimum technological condition was enzymolysis pH 4.0,enzyme addition 1.2％,enzymolysis temperature 35 ℃,enzymolysis time 2 h,ultrasonic power 540 W and ultrasonic time 4 min.Under this condition,the extraction rate of Dendrobium candidum polysaccharides could reach 39.23％.Dendrobium officinale polysaccharide had good scavenging ability on DPPH·and OH·,which indicated that Dendrobium officinale polysaccharide had good antioxidant activity.
Dendrobium officinalepolysaccharidesultrasonic and enzyme-assistedantioxidant activity
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