摘要
为研究市售泡椒鸡爪污染菌中是否存在新冠病毒相关同源基因,采集11份市售泡椒鸡爪等熟肉制品,经DNA提取、测序、原始序列质控、物种注释、KEGG数据库比对发现,11个样品中检出红苍白草螺菌、肺炎克雷伯菌、泛菌属、禽白血病毒与禽逆转录酶病毒,分别具有核糖体小亚基蛋白S14e、核糖体小亚基蛋白S3Ae、核糖体小亚基蛋白S2e、表皮生长因子受体(EC:2.7.10.1)、原癌基因蛋白c-fos,与新冠病毒KEGG生物通路高度关联,其中表皮生长因子受体(EC:2.7.10.1)在禽白血病毒与冠状病毒中均有检出,说明2种病毒均可作用于宿主表皮.原癌基因蛋白c-fos在禽逆转录酶病毒与新冠病毒的检出,说明2种病毒均能导致DNA过度表达.
Abstract
In order to study whether there was COVID-19 related homologous gene in the contaminated bacteria of pickled pepper chicken feet on the market,a total of 11 pieces of cooked meat products such as pickled pepper chicken feet on the market were collected.After DNA extraction,sequencing,original sequence quality control,species annotation and KEGG database comparison,the results showed that Spirillum rubrum,Klebsiella pneumoniae,Pantrichaea,Avian white blood virus and Avian reverse transcriptase virus were detected in 11 pickled pepper chicken feet,respectively,with ribosomal small subunit protein S14e,ribosomal small subunit protein S3Ae,ribosomal small subunit protein S2e,epidermal growth factor receptor(EC:2.7.10.1),proto oncogene protein c-fos and COVID-19 KEGG biological pathway.The epidermal growth factor receptor(EC:2.7.10.1)was detected in both avian leukemia virus and coronavirus,indicating that both viruses could act on the host epidermis.The detection of proto oncogene protein c-fos in avian retrovirus and COVID-19 indicated that both viruses could cause overexpression of DNA.
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