Simultaneous Determination of Dehydroacetic Acid and Bongkrekic Acid in Wet Rice Noodles and Wet Vermicelli by UPLC-MS/MS
Establish a method for simultaneous determination of dehydroacetic acid and bongkrekic acid in wet rice noodles and wet vermicelli by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).After the samples are extracted with 0.2％ammonia-80％methanol solution,the supernatant is obtained and passed through 0.22 μm microporous membrane,then separated with Agilent Infinity Lab Poroshell 120 EC-C18 column(2.1 mm×50 mm,1.9 μm),using 5 mmol/L ammonium acetate and methanol as mobile phase,detected by the electrospray ionization(ESI)negative ion multiple reaction monitoring mode,and quantified by external standard method.The results show that under optimized condition,dehydroacetic acid and bongkrekic acid have a good linear relationship in the range of 1-100 mg/L and 1-100 μg/L,respectively,and the detection limits of the method are 0.092 mg/kg and 1.8 μg/kg,respectively.The recovery rates of dehydroacetic acid at spiked levels of 4.0,20.0,and 80.0 mg/kg are 88.0％-99.8％,and the relative standard deviation(n=6)is 1.0％-3.4％.The recovery rates of bongkrekic acid at spiked levels of 8.0,100,and 240 μg/kg are 89.8％-106.1％,and the relative standard deviation(n=6)is 1.4％-2.5％.This method is simple,rapid,convenient,repeatable,sensitive and accurate,and can simultaneously detect dehydroacetic acid and bongkrekic acid in wet rice noodles and wet vermicelli.
ultra-performance liquid chromatography-tandem mass spectrometrydehydroacetic acidbongkrekic acidwet rice noodleswet vermicelli
NETL
NSTL
万方数据
