Ultrasound-Assisted Extraction of Organic Acids from Sea Buckthorn and Analysis of Their Components
The organic acids in sea buckthorn were extracted by ultrasonic assisted extraction.Explore the effects of ultrasonic time,liquid/feed ratio,ultrasonic power and volume fraction of ethanol on the yield of the total organic acids extracted.The optimal extraction conditions were obtained by response surface optimization.A comparison of the organic acids'types and contents in ultrasonic-assisted extracts was conducted.The results showed that the perfect circumstances for ultrasound-assisted extraction were 30 minutes of ultrasound,a liquid/feed ratio of 1∶15(g/mL),640 W of ultrasound power and 75% ethanol volume fraction under which a total organic acid yield was 37.66 mg/g.The main organic acids in the extract were determined by high-performance liquid chromatography(HPLC),and a total of 6 oxalic acids were extracted,namely oxalic acid,tartaric acid,malic acid,ascorbic acid,lactic acid and citric acid being detected.Of these,tartaric acid had the greatest concentration(14.45 mg/g),followed by malic acid(13.11 mg/g).This study provided a theoretical basis for the efficient utilization of sea buckthorn resources and the development of related products.
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