超声-微波辅助提取橄榄苦苷及抗氧化活性研究
Study on Extraction and Antioxidant Activity of Oleuropein from Olive Leaves by Synergistic Ultrasonic/Microwave Assisted
杨元萍 1赵冰馨 1王欣宇 1毛杨洋 1黎先发1
作者信息
- 1. 西南科技大学生命科学与工程学院(绵阳 621000)
- 折叠
摘要
以油橄榄叶为原料,研究超声-微波协同提取(UMAE)橄榄苦苷的最佳工艺.选用乙醇作为提取剂,并运用紫外-可见光谱差减法定量分析橄榄苦苷含量.通过单因素试验,选择提取时间、微波功率、固液比、温度和乙醇体积分数考察其对提取橄榄苦苷含量的影响,进行Plackett-Burman(PB)试验,得到超声-微波协同提取的关键工艺参数,并进行响应面分析(RSA).通过体外抗氧化试验,考察橄榄苦苷对DPPH、ABTS+和羟自由基的清除率效果.结果表明:提取时间18 min、微波功率475 W、固液比1∶45 g/mL、温度40 ℃、乙醇体积分数60％是超声-微波协同提取的最佳提取条件,此时橄榄苦苷含量为68.91±0.64 mg/g.DPPH自由基清除率的半抑制浓度(IC50)是0.035±0.001 mg/mL,羟自由基清除率的半抑制浓度(IC50)是0.004±0.010 mg/mL,ABTS自由基清除率的半抑制浓度(IC50)是0.058±0.003 mg/mL,表明油橄榄叶提取液具有较强抗氧化作用.
Abstract
The optimum process for ultrasonic-microwave synergistic extraction(UMAE)of oleuropein was investigated using olive leaves as raw material.Ethanol was selected as the extraction solvent,and the content of oleuropein was determined using differential subtraction method of UV-Vis spectroscopy.The effects of the independent variables time,microwave power,solid-liquid ratio,temperature and ethanol concentration on the content of oleuropein were investigated through one-way experiments.The Plackett-Burman(PB)test was performed to obtain the key process parameters in the ultrasound-microwave synergistic extraction method,and finally,response surface analysis(RSA)was performed.On this basis,the effect of oleuropein on the scavenging rate of DPPH,ABTS+andhydroxyl radicals was examined by in vitro antioxidant test.The results showed that the extraction time of 18 min,microwave power of 475 W,solid-liquid ratio of 1∶45 g/mL,temperature of 40 ℃,and ethanol concentration of 60% were the optimal condition for the ultrasound-microwave synergistic extraction,at which the content of oleuropein was 68.91±0.64 mg/g.The semi-inhibitory concentration(IC50)for the scavenging rate of the DPPH radicals was 0.035±0.001 mg/mL,the semi-inhibitory concentration(IC50)for ABTS radical scavenging was 0.058±0.003 mg/mL,and the semi-inhibitory concentration(IC50)for hydroxyl radical scavenging was 0.004±0.010 mg/mL.The study showed that olive extract obtained from olive leaves had strong antioxidant effect.
关键词
超声-微波协同提取/橄榄苦苷/响应面分析/抗氧化活性Key words
ultrasound-microwave assisted extraction/oleuropein/response surface analysis/antioxidant activity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据