Immunomodulatory Effects of Drug-Food Homologous Prescriptions on RAW 264.7 Macrophages
Three different formulations were prepared with red ginseng,Ejiao,Tuckaia,yam and wolfberry as RAW materials,and their immunomodulatory activity and mechanism on mouse mononuclear macrophages(RAW 264.7 cells)were investigated.Cell Counting Kit-8(CCK-8)was used to detect the proliferation of RAW 264.7 cells under different concentrations of preparations.The effects of drug-food homologous prescriptions on the phagocytic capacity,nitric oxide(NO),reactive oxygen species(ROS),interleukin-1β(IL-1β),interleukin-6(ILL-6)and tumor necrosis factor(TNF-α)cytokine secretion were determined by enzyme-linked immunoassay(ELISA).At the concentration of 50-200 μg/mL,drug-food homologous prescriptions had no toxic effect on RAW 264.7 macrophages.The results of ELISA experiments showed that the preparation of 200 μg/mL could significantly promote RAW 264.7 macrophages'NO-producing capacity(P＜0.05).In addition,the preparation promoted the phagocytosis ability,intracellular ROS production and cytokine secretion of RAW 264.7 macrophages to varying degrees.Using network pharmacology,a PPI network diagram of the intersection of ingredients and disease target genes was constructed.The results showed that drug-food homologous prescription could enhance immune function by regulating targets such as AKT1,STAT3,TNF,EGFR,SRC,ESR1 and MMP9.It demonstrated that the drug-food homologous prescriptions all exerted immunomodulatory effects by enhancing immune responses,and they exhibited characteristics of multiple components,multiple targets,and multiple pathways.
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