基质辅助激光解吸电离飞行时间质谱用于抗生素分析
Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry for Antibiotic Analysis
陈秀英 1王磊 1郑百芹 1丁琛2
作者信息
- 1. 唐山市食品药品综合检验检测中心(唐山 063000)
- 2. 河北北方学院农林科技学院(张家口 075032)
- 折叠
摘要
结合非晶态聚N-乙烯基咔唑(PVK)和兼具基质和吸附剂功能的单层氧化石墨烯(SLGO)优点,制备复合材料PVK@SLGO作为MALDI-TOF MS基质用于喹诺酮类抗生素正、负双离子模式下检测.通过引入同位素内标ENR-D5,建立一种快速便捷MALDI-TOF MS定量分析牛奶样品中ENR方法,喹诺酮类抗生素在0.000 1～1.0 mg/mL浓度范围内具有良好线性关系(R2=0.999 1),采用0.005,0.01和0.1 mg/mL添加水平的回收率范围在100.7％～113.6％之间,相对标准偏差为9.05％～14％(n=3),该方法的检出限为0.000 1 mg/mL,定量限为0.005 mg/mL.该方法具有良好的准确性和精密度,抗干扰能力强,灵敏度高,为快速分析食品中抗生素残留提供一种通用方法.
Abstract
The advantages of amorphous poly(N-vinylcarbazole)(PVK)and monolayer graphene oxide(SLGO),which has both matrix and adsorbent functions,are combined to prepare composite materials PVK@SLGO as matrix assisted laser desorption ionization mass spectrometry(MALDI-TOF MS)matrix.It was used for the detection of quinolone antibiotics in positive and negative double ion mode.In addition,by introducing the isotope internal standard enrofloxacin-D5(ENR-D5),a rapid MALDI-TOF MS method for the quantitative analysis of enrofloxacin(ENR)in milk samples was established,which had a good linear relationship in the concentration range of 0.000 1-1.0 mg/mL(R2=0.999 1).The recoveries at the addition levels of 0.005,0.01 and 0.1 mg/mL are in the range of 100.7% to 113.6% ,with the relative standard deviations of 9.05% to 14% (n=3).The limits of detection and the limits of quantification were 0.000 1 and 0.005 mg/mL respectively.This method had good accuracy and precision,strong anti-interference ability and high sensitivity.A general method for rapid analysis of antibiotic residues in food is provided.
关键词
传统基质辅助激光解吸电离质谱(MALDI-TOF/MS)/复合材料PVK@SLGO/抗生素/基质Key words
matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS)/composite material PVK@SLGO/antibiotic/matrices引用本文复制引用
出版年
2024
NETL
NSTL
万方数据