Detection of VC Palmitate Content in Winterized Oil
To analyze the addition of VC palmitate in winterized oil quantitatively,establish a high-performance liquid chromatography method for determining the content of VC palmitate added in winterized oil.Use methanol containing 1 mg/mL citric acid as the sample extraction agent,use methanol-water-acetic acid(95∶5∶0.1,V/V)as the mobile phase.Flow rate is 1 mL/min,After equal elution on a reversed phase C18 chromatographic column and detection with a UV detector,the optimal absorption wavelength is 250 nm.The results indicate that the qualitative detection limit of the method is 0.5 mg/kg,and the quantitative limit is 1.5 mg/kg.The recovery rate of spiking is between 88.18% and 101.54% ,SRSD is between 1.24% and 6.97% .This method can quickly and efficiently determine the content of VC palmitate in winterized oil without special solvents and complex reactions.
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