Study on Immobilized Lysozyme with Molecular Sieve SBA-15
Lysozyme(Lyz)was adsorbed with molecular sieve SBA-15 as a fixed support to prepare SBA-15-LYZ.The effects of lysozyme concentration,temperature,pH,Na+concentration of the medium and adsorption time on lysozyme fixation by SBA-15 were investigated.SBA-15-Lyz was characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FTIR)and thermogravimetry,and its thermal stability and pH stability were determined.The results showed that the fixation capacity of SBA-15 to lysozyme was positively correlated with the concentration of lysozyme medium,adsorption time and temperature,and negatively correlated with salt concentration.The maximum fixed capacity was 691.04 mg/g at 35 ℃.The adsorption behavior of SBA-15 to lysozyme was consistent with Langmuir model(R2=0.978 6)and quasi-second-order kinetic model(R2=0.999 9).After incubating the immobilized enzyme at 100 ℃ and pH 10.0 for 1 h,it was found that the immobilized enzyme had stronger thermal stability and pH stability than the free enzyme,with the relative enzyme activity retaining 43.40％and 84.76％,respectively,which was conducive to broaden the lysozyme application in food sterilization industry.
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