分子筛SBA-15固定化溶菌酶的研究
Study on Immobilized Lysozyme with Molecular Sieve SBA-15
陆鼎 1王亮 2李立 1邱伟强 3冯宇辉 4金银哲1
作者信息
- 1. 上海海洋大学食品学院(上海 201306);食品热加工工程中心(上海 201306)
- 2. 上海海洋大学食品学院(上海 201306);美国艾地盟公司(迪凯特60601)
- 3. 上海海洋大学食品学院(上海 201306)
- 4. 吉林烟草工业有限责任公司(延吉 136200)
- 折叠
摘要
用分子筛SBA-15作为固定载体吸附溶菌酶(Lysozyme,Lyz)制成功能化材料SBA-15-Lyz.研究介质的溶菌酶浓度、温度、pH、Na+浓度和吸附时间对SBA-15固定溶菌酶的影响.利用扫描电镜、透射电镜、傅里叶变换红外光谱和热重仪对SBA-15-Lyz进行表征,并测定其热稳定性与pH稳定性.结果显示:SBA-15对溶菌酶的固定容量与溶菌酶介质的浓度、吸附时间以及温度呈正相关,与盐离子浓度呈负相关.温度为35 ℃时达到最大固定容量691.04 mg/g.SBA-15吸附溶菌酶的行为符合Langmuir模型(R2=0.978 6)与准二级动力模型(R2=0.999 9).将固定酶在100 ℃和pH 10.0的条件下分别孵育1 h后发现,固定酶具有比游离酶更强的热稳定性与pH稳定性,相对酶活分别保留43.40％和84.76％,有利于拓宽溶菌酶在食品杀菌领域的应用范围.
Abstract
Lysozyme(Lyz)was adsorbed with molecular sieve SBA-15 as a fixed support to prepare SBA-15-LYZ.The effects of lysozyme concentration,temperature,pH,Na+concentration of the medium and adsorption time on lysozyme fixation by SBA-15 were investigated.SBA-15-Lyz was characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FTIR)and thermogravimetry,and its thermal stability and pH stability were determined.The results showed that the fixation capacity of SBA-15 to lysozyme was positively correlated with the concentration of lysozyme medium,adsorption time and temperature,and negatively correlated with salt concentration.The maximum fixed capacity was 691.04 mg/g at 35 ℃.The adsorption behavior of SBA-15 to lysozyme was consistent with Langmuir model(R2=0.978 6)and quasi-second-order kinetic model(R2=0.999 9).After incubating the immobilized enzyme at 100 ℃ and pH 10.0 for 1 h,it was found that the immobilized enzyme had stronger thermal stability and pH stability than the free enzyme,with the relative enzyme activity retaining 43.40％and 84.76％,respectively,which was conducive to broaden the lysozyme application in food sterilization industry.
关键词
SBA-15/溶菌酶/固定化/等温吸附模型/吸附动力模型/酶活稳定性Key words
SBA-15/lysozyme,immobilization/isothermal adsorption model/adsorption kinetic model/enzyme activity stability引用本文复制引用
出版年
2024
NETL
NSTL
万方数据