Optimization of Extraction Method for Crude Protein in Large Leaf Autumn Tea and Its Practicality
Explore the efficient extraction method of crude protein from Lincang ancient tree autumn tea,and study the influence of geographical factors on the extraction rate of crude protein,so as to improve the value utilization of tea resources.The protein extraction was performed by the basic acid sedimentation method,and the extraction process was optimized,with the focus on the influence of extraction conditions,and from a commercial perspective to obtain the best results.The studies showed that pH extraction had a significant effect on the extraction results,while the change in the extraction time had less effect on the results.Under the optimized extraction condition of extraction solution ratio 1∶30 g/mL,extraction temperature 50 ℃,extraction time 90 min,and extraction pH 7.5,the excellent results were obtained,and the protein extraction rate was as high as 44.24％.By optimizing the extraction process,the extraction rate of crude protein is improved,and the significant savings of energy,cost and production time are realized,which provides a basis for the high value utilization of ancient tree autumn tea,and is expected to contribute to the sustainable development of the tea industry.
ancient tree teacrude proteinprotein extractionvalue utilization
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