Determination of β-Nicotinamide Mononucleotide and Nicotinamide Adenine Dinucleotide in capsule
A method for simultaneous determination of β-nicotinamide mononucleotide(NMN)and nicotinamide adenine dinucleotide(NAD+)in capsule by HPLC(high performance liquid chromatography)was established.The sample was extracted with Phosphate buffer(PBS),and ZORBAX SB-Aq chromatographic column was used for gradient elution with 0.1％(V/V)formic acid and methanol and water as mobile phase.It was detected by ultraviolet detector and quantified by external standard.Under the optimized condition,NMN and NAD+had a good linear relationship in the concentration range of 1.00-100 mg/L,and the correlation coefficients of them were 0.999 9 and 0.999 9,respectively.The limits of detection of NMN and NAD+were 0.1 g/kg,while the limits of quantitation were 0.3 g/kg.The recoveries of NMN and NAD+ranged from 81.6％to 101％at the concentration level of 0.3,0.6 and 3.0 g/kg,and the relative standard deviations were 0.737％-2.15％.The method had the advantages of high sensitivity,good selectivity,accurate and reliable results and simplicity of operator,which was suitable for the simultaneous quantitative analysis of NMN and NAD+in capsule.
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