Community Structure of Crayfish(Procambarus clarkii)Intestine Microbiota Based on High-Throughput Sequencing Technology
The intestinal microflora of Procambarus clarkii was analyzed using high-throughput sequencing technology to investigate the impact of sex.The results revealed that the diversity index of female Procambarus clarkii's intestinal bacteria was higher compared to males,while the intestinal fungal diversity index showed an opposite trend.At the phylum level,dominant bacterial groups in both females and males included Tenericutes,Proteobacteria,Firmicutes,Bacteroidetes,etc.Female Procambarus clarkii exhibited dominance in Lachnospira,Anaerorhabdus,Bacteroides,Clostridium at the genus level.Uliginosibacterium,Clostridium,Bacteroides,and Anaerorhabdus were dominant bacterial groups in male Procambarus clarkii;However,the abundance varied significantly among individuals.As for fungi at the phylum level in both sexes of crayfish:Ascomycota,Basidiomycota,Mortierellomycota were dominant groups observed.Female Procambarus clarkii displayed dominance in Coprinopsis,Aspergillus,Fusarium at genus level.Male Procambarus clarkii,on the other hand,demonstrated dominance with Aspergillus,Gibberella,Fusarium;However,the abundance also varied greatly among individuals.These findings contribute to a better understanding of composition and succession patterns within intestinal flora of Procambarus clarkii as well as exploring functions played by these intestinal microorganisms during growth and development.
Procambarus clarkiiintestinemicrobial community structurehigh-throughput sequencingsex