Optimization of Extraction Process of Walnut Quinone from Walnut Green Husk by Response Surface Methodology
To study the content of walnut quinone in walnut green husk from different areas and to develop the extraction process of walnut quinone,this study collected green walnuts from different production areas in Xinjiang and Handan regions,and screened the extraction process of effective component walnut quinone in walnut green husk from the aspects of extraction solvent,extraction method,and drying method.Response surface analysis was used to design experiments to optimize the extraction process conditions of walnut quinone,and high-performance liquid chromatography was used to detect the content of walnut quinone.The results showed that the optimal solvent for extracting walnut quinone from walnut green husk was ethyl acetate,the optimal extraction method was water bath heating extraction,and the optimal drying method was freeze-drying.The optimal extraction process parameters are extraction temperature of 36 ℃,extraction time of 30 minutes,and material liquid ratio of 1∶11(g/mL).Under these conditions,the content of walnut quinone is 1.8％,and the extraction rate is 59.45％.After purification with ethyl acetate,the content of this crude extract can be increased to 8.35％.
walnut green huskwalnut quinoneextraction rateresponse surfacestability
万方数据
维普
