Optimization of Extracting Polyphenols from Apple Pomace
In order to study the best technology of extracting polyphenols from apple by ultrasonic-assisted composite enzyme,using apple residue as raw material,Plackett-Burman combined with Box-Behnken response surface method was used to investigate the effects of seven factors on the extraction rate of apple polyphenols,including the mass ratio of pectinase to cellulase,the addition amount of complex enzyme,enzymolysis time,liquid to solid ratio,ethanol volume fraction,ultrasonic power and ultrasonic time.The results showed that the optimal extraction condition was that mass ratio of pectinase to cellulase 1∶3,enzyme dosage 2.4％,enzymolysis time 81 min,liquid-solid ratio 25∶1(mL/g),ethanol volume fraction 75％,ultrasonic power 90 W and ultrasonic time 27 min.Under the extraction condition,the predicted value of apple polyphenol was 2.161 mg/g,and the final yield was 2.210 mg/g.The results can provide experimental basis for the effective utilization of apple pomace and the efficient extraction of apple polyphenols,further research and application.
apple pomaceapple polyphenolspectinasecellulaseultrasound
万方数据
维普
