Optimization of Extraction Process and Antioxidant Activity of Total Flavonoids from Tomato Pomace by Response Surface Methodology
This study was based on the response surface method to optimize the extraction process of total flavonoids from tomato pomace,the total flavonoids and polysaccharides from tomato pomace were isolated and purified by macroporous resin,and their antioxidant activity was evaluated.The results showed that the optimum extraction conditions of total flavonoids from tomato pomace were 90％ethanol,1∶10(g/mL)solid-liquid ratio,150 W ultrasonic power,extraction temperature of 60 ℃ and extraction time of 60 min.The content of total flavonoids in tomato pomace could reach 7.35 mg/g under these conditions,and the extraction rate was 0.735％.The optimal technological conditions for simultaneous separation and purification of total flavonoids and polysaccharides from tomato pomace by macroporous resin were as follows:AB-8 macroporous resin was used,the sample concentration of crude extract was 45 mg/mL,and the sample flow rate of crude body fluid was 2 mL/min;During elution,3 BV of distilled water was used to enrich polysaccharides,and 2 BV of 80％ethanol was used to enrich total flavonoids.It could be seen from the results that the content of total flavonoids and polysaccharides in the crude extract was significantly increased after separation and purification by macroporous adsorption resin.The content of total flavonoids increased from 7.35 mg/g to 53.6 mg/g,and the content of polysaccharides increased from 43.1 mg/g to 251.4 mg/g.The activity studies had showed that the total flavonoids had an ABTS free radical scavenging rate of 67.1％.The scavenging rate of polysaccharides on ABTS free radicals reached 36.8％.The antioxidant activity of polysaccharides and total flavonoids after separation and purification was significantly higher than that of crude extracts.This study provides a research basis for the discovery and preparation of functional components from tomato pomace,and provides a new way for the high-value utilization of tomato pomace.
tomato pomacetotal flavonoidspolysaccharidesseparation and purificationantioxidant activities
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