Determination of 4 Succinate Dehydrogenase Inhibitor Fungicides in Vegetable Oils
A method was developed for the fast determination of bixafen,isofetamid,luxapyroxad and penthiopyrad residues in vegetable oils by ultra-high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)coupled with modified QuEChERS.Samples were extract with acetonitrile,and then the supernatant was purified with primary secondary amine(PSA)and octadecyl trimethoxysilane powder(C18).The 4 compounds were separated on a C18 column by gradient elution using methanol-0.1％(V/V)formic acid in water as the mobile phase after filtered through a 0.22 μm membrane,detected by UPLC-MS/MS,and quantified by the external standard method.The limits of detection and limits of quantitation for the method were 0.002 mg/kg and 0.005 mg/kgrespectively.The calibration curves for all analytes showed good linearity in the range of 1-200 ng/mL.The recoveries of the four analytes spiked at three levels(0.005,0.010 and 0.020 mg/kg)in different matrices(soybean oil,peanut oil,corn oil,rapeseed oil,olive oil)ranged from 78.6％to 118.2％with the relative standard deviations(SRSD,n=6)from 0.8％-9.6％.The method was easy and fast,and showed good purification efficiency and the results are accurate,which is suitable for the determination of succinate dehydrogenase inhibitor fungicides in vegetable oils.
ultra-high performance liquid chromatography-tandem mass spectrometryvegetable oilsuccinate dehydrogenase inhibitor fungicides
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