To establish an analysis method for the determination of oxyphenisatin dipropionate in weight loss compression candies by ultra performance liquid chromatography tandem mass spectrometry,the samples were extracted by 80％methanol water solution sonication.Then,waters BEH-C18(2.1 mm× 100 mm,1.7 μm)was adopted with the mobile phase of acetonitrile-ammonium acetate aqueous solution(5 mmol/L)in gradient elution.The flow rate was 0.4 mL/min,the sample volume was 2 μL,and the temperature of column was 40 ℃,thereafter,ionized in the positive ion mode,detected by multi-reaction monitoring and quantified by external standard.The results showed that the standard curves of oxyphenisatin dipropionate in the range of 0.05,1.0,5.0 and 10.0 mg/kg(n=6)showed good linearity,and the correlation coefficient r was 0.999 5.The average recoveries of oxyphenisatin dipropionate were 87.6％-89.7％in range of 0.05-10.0 mg/kg addition,and the relative standard deviation was 2.42％-2.61％.The detection limit and quantification limit were 0.010 mg/kg and 0.030 mg/kg,respectively.The method is simply,rapidly,highly accurate and accuracy,which is suitable for the determination of large quantities of samples with strong practical value.
关键词
UPLC-MS/MS法/减肥类压片糖果/双丙酚丁
Key words
UPLC-MS/MS/weight loss compression candies/oxyphenisatin dipropionate
维普
万方数据