A more efficient and accurate dual-wavelength colorimetric method was used to determine the ratio of amylopectin to total starch in sorghum.Compared with the traditional single-wavelength colorimetric method or dual-wavelength colorimetric method,the pretreatment time of samples was shortened and the indirect test steps were reduced.In the experiment,amylopectin standard solution and amylopectin standard solution were fully scanned in the wavelength range of400-900 nm,and 535 nm and 630 nm wavelength with large absorbance were selected to measure the proportion of amylopectin in sorghum.The recovery rate(R)was verified.The average recovery rate was 98.64％,and the relative standard deviation of recovery rate was 2.17％.The standard deviation(S)was 0.16％,the coefficient of variation(C.V.)was 0.16％,the detection limit(SLOD)was 4.15％,and the limit of quantification(SLOQ)was 8.70％.This method was quick and accurate to determine the proportion of amylopectin in sorghum,and could provide a basis for rapid judgment of sorghum quality.
维普
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