A method for the determination of fructooligosaccharide content in infant formula milk powder was established using ion chromatography-pulsed amperometric detector.The sample was extracted with hot water at 80 ℃ for 15 min.The sample solution was directly digested by fructanase(enzymatic hydrolysis temperature 40 ℃,enzymatic hydrolysis time 45 min,boiling water bath 5 min),separated by CarboPac PA10 column,using a mobile phasecomposed of a mixture of sodium hydroxide and water by gradient elution,detected by pulsed amperometric.Fructose calibration curves were linear in the concentration range of 0.200 0-10.00 mg/L,R=0.999 9.The quality control sample of QC-IP-715 results was accurate and relative standard deviation of 0.37％(n=6).The relative standard deviation between the measured value of fructooligosaccharides and the labeled value of 30 batches of infant formula milk powder on the market was between 0.4％and 8.4％.The method was efficient,accurate,reliable,and thus suitable for the determination of fructooligosaccharide content in infant formula milk powder.
维普
万方数据