Optimization of Extraction and Antioxidant Properties of SOD From Chaenomeles speciosa
Selection of Chaenomeles speciosa as raw material,ultrasound was used to assist in the extraction of superoxide dismutase(SOD)from Chaenomeles speciosa.Orthogonal experiment of four factors and three levels was used to optimize the extraction process parameters of SOD in Chaenomeles speciosa,with the pH,solid-liquid ratio,extraction time and ultrasonication time as test factors and SOD viability as experimental indexes.Meanwhile,we initially investigated the antioxidant activity of SOD in Chaenomeles speciosa,including scavenging activity on OH radical and the protective effect of SOD on the oxidative damage in primary mouse jejunal mucosal epithelial cells induced by H2O2.The cells were divided into the H2O2+SOD group(60 mg/mL SOD pretreatment for 24 h,3％H2O2 treatment for 24 h)and the H2O2 group(3％H2O2 treatment for 24 h).The cell activity was detected by Calcein AM/PI staining.The results showed that the best extraction conditions were phosphate buffer pH 6.8,solid-liquid ratio 1︰6(g/mL),extraction time 2 hours,and ultrasound time 40 minutes,the enzyme activity of SOD in Chaenomeles speciosa was 289.28(U/g),and SOD in Chaenomeles speciosa had strong hydroxyl radical scavenging(97.26％,1 mg/mL)and an obvious protective effect on H2O2-induced oxidative stress damage in primary mouse jejunal mucosal epithelial cells.
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