Process Optimization of Casein Hydrolysis by Alkaline Protease
The optimal process conditions of casein hydrolysis by alkaline protease were studied.On the basis of single factor experiment,according to the principle of Box-Behnken central composite experimental design,the response surface analysis method of four factors and three levels was used,and the degree of hydrolysis was used as the response surface and contour line.The optimum conditions for the hydrolysis of casein by alkaline protease were as follows:alkaline protease addition amount 3％,substrate concentration 5％,hydrolysis time 254 min,hydrolysis pH 7.5 and hydrolysis temperature 55 ℃,and the degree of hydrolysis was up to 35.02％.
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