Assessment of the Sleep-Improving Efficacy of Yak Milk Powder Based on a Zebrafish Insomnia Model
Evaluate the effect of whole yak milk powder on sleep in a zebrafish insomnia model.Zebrafish were randomly divided into 6 groups,including normal control group,model control group(pentylenetetrazol,PTZ),melatonin group,and different quality concentration groups of whole yak milk powder(low-dose group,medium-dose group,and high-dose group).The maximum tolerated concentration(MTC)was used to screen for the maximum effective concentration of whole yak milk powder.Zebrafish arousal activity and total arousal time were determined using ethological behavior analysis.The relative expression levels of mtnr1aa and gabra1 genes were detected using q-PCR.The results suggested that the maximum effective concentration of whole yak milk powder was 2 000 μg/mL.Under its intervention,the arousal activity and total arousal time of zebrafish were significantly reduced,and the relative expression levels of mtnr1aa and gabra1 genes were significantly upregulated.Whole yak milk powder has a sleep-promoting effect on PTZ-induced zebrafish insomnia,but its specific mechanism of action requires further investigation.
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