Comparison of Detection Methods for Monacolin K content in Monascus Products
Monacolin K(MK)is a common component in the fermentation products of Monascus.Although various quanti-tative techniques have been developed to detect MK in Monascus products,most require expensive equipment and specially trained personnel.Based on the different adsorption capacities of MK and impurities on fixed phases,as well as the differenceΔA in absorbance of MK at wavelengths of 246 nm and 254 nm,the relationship between absorbance difference and MK concentration was studied.Using 80％ethanol as the extractant for MK in Monascus fermentation broth,a comparative study was conducted on the accuracy of single wavelength UV detection and dual wavelength UV detection before and after chromatography for determining MK in red yeast samples.It was found that the method of using dual wavelength UV detection to detect MK in red yeast products after chromatography separation was highly accurate,with a correlation coefficient of0.925,a recovery rate of 104.56％±1.43％,a precision of 1.4％,and a minimum detection limit of 1.52 mg/kg.This method can replace the expensive high-performance liquid chromatography method,and can be used for quality control of Monascus fermentation products under general experimental conditions.
ultraviolet spectrophotometrydual-wavelengthMonascusmonacolin K
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