Uncertainty Evaluation for Determination of Acesulfame Potassium in Patisseriesamples by High Performance Liquid Chromatography
Evaluate the uncertainty of determination of acesulfame potassium in patisserie samples by high performance liquid chromatography(HPLC).According to GB 5009.140-2023 National Food Safety Standard Determination of Potassium Acetosulfonate in Food,the content of acesulfame in pastries has been determined.Guide to the evaluation and expression of uncertainty in measurement,the sources of uncertainty in HPLC determination of acesulfame potassium in patisserie samples have been fully analyzed.Furthermore,the combined uncertainties and expanded uncertainties have been calculated based on the established mathematical models.Results showed that The content of acesulfame potassium in patisserie could be shown as 5.86±0.11 mg/kg(k=2).The sources of uncertainty in the measurement process mainly include the standard stock solution,the configuration of standard curves,the fitting of standard curves,the determination of sample repeatability,the constant volume of the sample,the spiked recovery rate of the experiment and the repeatability testing.However,the uncertainty of the standard reserve solution itself contributes the most to the final synthesis uncertainty,and the factor of sample weighing has the smallest contribution.This method can reflect the confidence and accuracy of using high-performance liquid chromatography to determine acesulfame in pastries,and providing reference for daily practical inspection work.It provided reference for daily practical testing work.
high performance liquid chromatographypatisseriesamplesacesulfame potassiumthe uncertainty
NETL
NSTL
万方数据
