Simultaneous Determination of 9 Glucocorticoids in Animal-Derived Foods by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry with Stable Isotopic Labeling
Establish a method for the determination of 9 glucocorticoids in animal derived foods by stable isotope labeling-high performance liquid chromatography-tandem mass spectrometry.The sample was extracted with 1％formic acid in acetonitrile,and the extract was purified by pass-through solid phase extraction before being measured by liquid chromatography-tandem mass spectrometry.The internal standard method was used for quantification.The linear relationship of 9 glucocorticoids were good within their respective concentration ranges.The average recoveries ranged from 72.1％to 102.5％,and the relative standard deviations were between 1.4％and 9.3％(n=6).This method had the characteristics of high sensitivity,good accuracy,simple operation and high detection efficiency.It could meet the detection needs of 9 glucocorticoids in animal-derived food.
high performance liquid chromatography-tandem mass spectrometryanimal-derived foodsglucocorticoidsstable isotope labeling
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