高效液相色谱-串联质谱法测定动物源性食品中9种糖皮质激素
Simultaneous Determination of 9 Glucocorticoids in Animal-Derived Foods by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry with Stable Isotopic Labeling
宋娟 1石培育 1陈燕秋 1吴雪莹 1陈世奇 2戴琴1
作者信息
- 1. 成都市食品检验研究院(成都 611130);国家市场监管重点实验室(营养与健康化学计量及应用)(北京 100029)
- 2. 国家市场监管重点实验室(调味品监管技术)(重庆 401102)
- 折叠
摘要
针对不同动物源性食品中食源性兴奋剂检测基质效应差异大、干扰多等问题,建立稳定同位素标记-高效液相色谱-串联质谱法测定动物源性食品及其制品中9种糖皮质激素的方法.试样经1％甲酸乙腈提取,通过式固相萃取柱净化,采用液相色谱-质谱/质谱仪测定,同位素内标法定量.9种糖皮质激素在各自浓度范围内线性关系良好,平均回收率为72.1％～102.5％,相对标准偏差为1.4％～9.3％(n=6).该方法具有灵敏度高、准确性好、操作简单、检测效率高等特点,提高不同复杂基质下的测量准确性,能够满足多种动物源性食品及其制品中9种糖皮质激素的检测需求.
Abstract
Establish a method for the determination of 9 glucocorticoids in animal derived foods by stable isotope labeling-high performance liquid chromatography-tandem mass spectrometry.The sample was extracted with 1％formic acid in acetonitrile,and the extract was purified by pass-through solid phase extraction before being measured by liquid chromatography-tandem mass spectrometry.The internal standard method was used for quantification.The linear relationship of 9 glucocorticoids were good within their respective concentration ranges.The average recoveries ranged from 72.1％to 102.5％,and the relative standard deviations were between 1.4％and 9.3％(n=6).This method had the characteristics of high sensitivity,good accuracy,simple operation and high detection efficiency.It could meet the detection needs of 9 glucocorticoids in animal-derived food.
关键词
高效液相色谱-串联质谱/动物源性食品及其制品/糖皮质激素/稳定同位素标记Key words
high performance liquid chromatography-tandem mass spectrometry/animal-derived foods/glucocorticoids/stable isotope labeling引用本文复制引用
出版年
2024
NETL
NSTL
万方数据