Simultaneous Determination of Aflatoxins B1,B2,G1 and G2 in Edible Oil by HPLC-MS/MS
Determine aflatoxins B1,B2,G1,G2 in edible oil by High-performance liquid chromatography tandem mass spectrometry.This method extracts aflatoxins B1,B2,G,and G2 from the sample by methanol water solution(70+30,V/V),the extraction solution was diluted with PBS solution containing 1％Tween-20,purified and enriched through an immunoaffinity column.After concentration,constant volume and filtration of the purified liquid,it was separated by liquid chromatography,detected by tandem mass spectrometry and quantified by isotope internal standard method.The results showed that the linear relationship among the aflatoxins B1,B2,G1 and G2 was good in the concentration range of 0.1-10.0 ng/mL.The linear correlation coefficients were greater than 0.99.When the detection limit of the method was 0.03 μg/kg,the response values of all three compounds were greater than 3.When the limit of quantification of the method was 0.1 μg/kg,the response values of all three compounds were greater than 10.The recoveries were 73.0％-105.0％at the concentrations of 1.0,5.0 and 8.0 μg/kg,and the relative standard deviations were 1.24％-5.56％(n=7).The results meet the requirement of method verification.
high performance liquid chromatography-tandem mass spectrometryedible oilaflatoxins
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