HPLC-MS/MS法测定鸡肉中赛杜霉素含量的测量不确定度评定
Evaluation of Measurement Uncertainty in the Determination of Sedumycin in Chicken samples by HPLC-MS/MS
陶滔 1窦明理 1刘娜 1苏燕 1王明 1徐峰1
作者信息
- 1. 四川省食品检验研究院(成都 611731)
- 折叠
摘要
赛杜霉素作为治疗鸡球虫病的常见药物,其含量测定引起的关注不多,对于鸡肉中赛杜霉素含量的测量不确定度评定更是未见报道.采用液相色谱-串联质谱(HPLC-MS/MS)法对鸡肉中赛杜霉素含量进行测定,建立数学模型,使用鱼骨图进行测量不确定度来源分析,从标准溶液配制、样品的称量、样品前处理过程、仪器稳定性、样品重复性检测与回收率6个方面进行测量不确定度评定,根据贡献率对不确定度分量进行排序.结果表明,鸡肉中赛杜霉素添加水平为50.0 μg/kg时,其扩展不确定度为6.2 μg/kg,k=2,影响测量不确定度的主要来源为检验过程中标准溶液配制、重复性检测和回收率.
Abstract
As a common drug for the treatment of chicken coccidiosis,the determination of saidumycin has attracted little attention,and the evaluation of uncertainty in the determination of saidumycin in chicken meat has not been reported.The content of sedamycin in chicken was determined by liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),and a mathematical model was established.The source of measurement uncertainty was analyzed by fishbone diagram.The measurement uncertainty was evaluated from six aspects of standard solution preparation,sample weighing,sample pretreatment process,instrument stability,sample repeatability detection and recovery rate.The uncertainty components were sorted according to the contribution rate.The results showed that the expanded uncertainty was 6.2 μg/kg,k=2,when the addition level of Saidumycin in chicken was 50.0 μg/kg.The main sources affecting the measurement uncertainty were the preparation of standard solution,repeatability test and recovery rate during the test.
关键词
鸡肉/赛杜霉素/液相色谱-串联质谱法(HPLC-MS/MS)/测量不确定度Key words
chicken/sedumycin/liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)/measurement uncertainty引用本文复制引用
出版年
2024
NETL
NSTL
万方数据