Study on Antioxidant and Anti-inflammatory Activities of Polysaccharides from Morifructus by Enzyme-Assisted Extraction in Vitro
The scavenging rates of DPPH,OH,ABTS+·and superoxide anion free radical of different concentrations of Mori fructus polysaccharide(MFP)were determined by enzyme-assisted extraction.The LPS-induced inflammation model of Raw264.7 in vitro was established to explore the anti-inflammatory activity of MFP.The results showed when the concentration of MFP was 1.0 mg/mL,the scavenging rates of DPPH·,OH·,ABTS+·and superoxide anion free radical reached 88.74％,44.96％,81.35％and 56.38％,respectively.The results showed that there was no significant toxic effect on Raw264.7 cells when the concentration of MFP was 0-200 μg/mL.When the concentration of MFP was 200 μg/mL,the inhibition rates of NO,IL-6,IL-1β,TNF-α and COX-2 in Raw264.7 cells induced by LPS were 33.7％,42.9％,57.1％,55.3％and 56.9％,respectively.The above results showed that enzymatic assisted extraction of MFP had excellent antioxidant and anti-inflammatory activities in vitro,which was intended to lay a foundation for the development of natural functional food.
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