Study on Identification of Pu-erh Tea from Ancient Tea Plant Varied with Famous Tea Mountains Based on 1H-NMR
Identification model was established based on nuclear magnetic resonance hydrogen spectroscopy(1H-NMR)to distinguish Pu-erh tea of ancient tea plants from three famous tea mountains,namely Hekai ancient tea mountain,Bulang ancient tea mountain,and Yiwu ancient tea mountain in Xishuangbanna prefecture,Yunnan province.Totally 21 components were obtained by 1H-NMR,including five catechins(epigallocatechin gallate,epigallocatechin,epicatechin gallate,epicatechin and catechin),six amino acids(theanine,alanine,threonine,isoleucine,valine and leucine),four organic acids(quinic acid,acetate,fatty acid and gallic acid),four sugars(α-glucose,β-glucose,fructose and sucrose),caffeine and 2-O-arabopyranositol.Moreover,raw and ripe Pu-erh tea samples of the same tea mountain could be effectively distinguished by PCA-Class pattern discriminant analysis.Furthermore,the discrimination results of raw Pu-erh tea from different famous tea mountains showed that the accuracy of samples from Bulang ancient tea mountain was 100％,while the samples from Hekai and Yiwu ancient tea Mountains were misidentified.Thus,the model accuracy of discrimination was 58.33％.Therefore,1H-NMR could effectively distinguish Pu-erh tea from the same tea mountain varied with different processing techniques,however there were some limitations in distinguishing raw Pu-erh tea of ancient tea plants from different famous tea mountains in the same region.
Pu-erh tea from ancient tea plantsnuclear magnetic resonance hydrogen spectroscopyorigin identificationPCA-Class
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