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铁皮石斛-青钱柳复合饮品研制及体外抗氧化活性研究

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以铁皮石斛、青钱柳提取物为主要原料,添加柠檬酸、白砂糖、香兰素、果胶研制复合饮品。以感官评价为指标,采用单因素试验和响应面试验对复合饮品的配方进行优化,并对复合饮品的抗氧化性及各项指标进行检测。试验结果表明,该复合饮品的最佳配方为铁皮石斛汁18。52 mg/mL,青钱柳汁12。72 mg/mL,柠檬酸3。74 mg/mL,白砂糖0。07 mg/mL,感官评分为88。测定复合饮品中总多糖含量为1。85 mg/mL,总蛋白含量为0。06 mg/mL。通过体外抗氧化活性试验得出铁皮石斛-青钱柳复合饮品对DPPH自由基、羟自由基、超氧阴离子自由基的具有清除能力,IC50值分别为7。73,5。15和6。97 mg/mL。证明复合饮品具备一定抗氧化活性,但是其抗氧化活性低于维生素C。通过试验验证表明采用响应曲面优化铁皮石斛-青钱柳复合饮品配方可行,为铁皮石斛-青钱柳复合饮品产品的开发提供理论依据。
Development of Dendrobium officinale and Cyclocarya paliurus Compound Drink and Its Antioxidant Activity in Vitro
The extract of Dendrobium officinale and Cyclocarya paliurus was used as the main raw materials,and citric acid,white sugar,vanillin and pectin were added to develop a compound drink.Taking sensory evaluation as the index,single factor experiment and response surface experiment were used to optimize the formula of compound drinks.The antioxidant activity and various indexes of compound drinks were tested.The results showed that the optimal formula of the compound drink was 18.52 mg/mL of Dendrobium officinale juice,12.72 mg/mL of Cyclocarya paliurus juice,3.74 mg/mL of citric acid,and 0.07 mg/mL of white sugar,and sensory score was 88.The total polysaccharide content and total protein content in compound drinks were 1.85 mg/mL and 0.06 mg/mL.The results of in vitro antioxidant activity experiments showed that Dendrobium officinale-Cyclocarya paliurus compound drink had scavenging ability against DPPH free radicals,hydroxyl free radicals and superoxide anion free radicals,with IC50 values of 7.73,5.15 and 6.97 mg/mL,respectively.It is proved that compound drinks have certain antioxidant activity,but their antioxidant activity is lower than that of vitamin C.The experimental verification shows that it is feasible to optimize the formula of Dendrobium officinale-Cyclocarya paliurus composite beverage by using response surface,which provides a theoretical basis for the development of Dendrobium officinale-Cyclocarya paliurus composite beverage products.

Dendrobium officinaleCyclocarya paliuruscompound drinksconfused evaluationantioxidant

郑晓杰、鲁雲、张玉娇、董国锋、李彦坡、邓文彬

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温州科技职业学院(温州市农业科学研究院)/浙南作物育种重点实验室(温州 325006)

兰州理工大学生命科学与工程学院(兰州 730050)

浙江铁枫堂健康科技有限公司(温州 325006)

铁皮石斛 青钱柳 复合饮品 迷糊评价 抗氧化

2024

食品工业
上海市食品工业研究所

食品工业

影响因子:0.47
ISSN:1004-471X
年,卷(期):2024.45(11)