Preparation of Hydrophilic-Lipophilic Balance Polymer Microspheres and Its Application in Veterinary Drug Residue Analysis
In this work,two polymerization monomers,i.e.,divinylbenzene(DVB)and N-vinyl-2-pyrrolidone(NVP),as well as azobisisobutyronitrile(AIBN)as the initiator,were employed to prepare hydrophilic-lipophilic balance(HLB)polymer microspheres.During the polymerization process,toluene and cyclohexanol were used as porogen agents,and hydroxypropyl methylcellulose(HPMC)was used as a dispersant.The HLB microspheres were prepared through suspension polymerization method and characterized by scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS),N2 adsorption-desorption isotherm analysis,and infrared spectra.The home-made HLB microspheres were used as the packing material to prepare the solid-phase extraction(SPE)columns.Various veterinary drug residues from the livestock meat and poultry were extracted and purified by the HLB SPE columns according to GB 31658.17-2021 standard method,and high-performance liquid chromatography-tandem mass spectrometry was applied for veterinary drug analysis.The results showed that home-made HLB microspheres displayed good monodispersity,homogenous particle size,large specific surface area,uniform pore size distribution,and abundant polar and non-polar functional groups on the surface.Furthermore,the simultaneous extraction and purification of 33 kinds of veterinary drugs from livestock meat and poultry samples were achieved by using the home-made HLB microspheres packed SPE column.The recoveries ranging in 58.6％to 102.7％and the relative standard deviations(SRsD)within 0.1％to 5.2％were acquired by the home-made HLB SPE column,which were higher than those gained by the commercial HLB column(recovery:46.7％-87.6％.SRSD:0.1％-8.7％).This study clearly indicated the great potential of home-made HLB microspheres in the analysis of veterinary drug residues from complex sample matrices.
hydrophilic-lipophilic balance polymer microspheressuspension polymerizationsolid-phase extractionveterinary drug residuesliquid chromatography-tandem mass spectrometry
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维普
