食品工业2024，Vol.45Issue(11) ：54-59.

低共熔溶剂法提取葡萄皮籽多酚、黄酮及其抗氧化性研究

A Study on the Extraction of Polyphenols and Flavonoids from Grape Skin Seeds and Its Antioxidant Properties bv Deep Eutectic Solvent Process

何彦芳 陈敬书 郭欢欢 褚文慧 王康威 侯旭杰
食品工业2024，Vol.45Issue(11) ：54-59.
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  • 维普
  • 万方数据

低共熔溶剂法提取葡萄皮籽多酚、黄酮及其抗氧化性研究

A Study on the Extraction of Polyphenols and Flavonoids from Grape Skin Seeds and Its Antioxidant Properties bv Deep Eutectic Solvent Process

何彦芳 1陈敬书 1郭欢欢 1褚文慧 1王康威 2侯旭杰1
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作者信息

  • 1. 塔里木大学食品科学与工程学院/南疆特色农产品深加工兵团重点实验室(阿拉尔 843300)
  • 2. 塔里木大学信息工程学院(阿拉尔 843300)
  • 折叠

摘要

以和田红葡萄皮籽为原材料,采用低共熔溶剂法提取其多酚和黄酮类物质,并与80％乙醇为溶剂提取效果进行比较试验.以黄酮和多酚提取量为指标,经单因素试验和响应面优化试验,获得了最优的提取工艺条件,并分析了提取液对DPPH、ABTS+自由基的清除能力.结果表明,低共熔溶剂的组成为氯化胆碱-乳酸(1∶2),含水量为20％,最佳工艺条件为提取温度50℃、提取时间35 min、料液比1∶26(g/mL),此时黄酮平均提取量为57.46 mg/g,多酚平均提取量为79.81 mg/g.葡萄皮籽提取物表现出显著的抗氧化活性.

Abstract

This research utilized Hontan red grape peels and seeds as raw materials,and employed a deep eutectic solvent(DES)method to extract polyphenols and flavonoids,comparing the results with extraction using 80％ethanol as the solvent.Through single-factor experiments and response surface methodology optimization,the optimal extraction conditions were determined,and the scavenging ability of the extract on DPPH and ABTS+free radicals was analyzed.The results indicated that the optimal DES composition was choline chloride-lactic acid(1∶2)with a water content of 20％.The best extraction conditions were an extraction temperature of 50 ℃,an extraction time of 35 minutes,and a solid-to-liquid ratio of 1∶26(g/mL).Under these conditions,the average extraction yield of flavonoids was 57.46 mg/g,and the average extraction yield of polyphenols was 79.81 mg/g.The extract from grape peels and seeds exhibited significant antioxidant activity.

关键词

低共熔溶剂法/响应面优化试验/黄酮/多酚

Key words

deep eutectic solvent method/response surface optimization experiment/flavone/polyphenol

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出版年

2024
食品工业
上海市食品工业研究所

食品工业

影响因子：0.47
ISSN：1004-471X
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