This research utilized Hontan red grape peels and seeds as raw materials,and employed a deep eutectic solvent(DES)method to extract polyphenols and flavonoids,comparing the results with extraction using 80％ethanol as the solvent.Through single-factor experiments and response surface methodology optimization,the optimal extraction conditions were determined,and the scavenging ability of the extract on DPPH and ABTS+free radicals was analyzed.The results indicated that the optimal DES composition was choline chloride-lactic acid(1∶2)with a water content of 20％.The best extraction conditions were an extraction temperature of 50 ℃,an extraction time of 35 minutes,and a solid-to-liquid ratio of 1∶26(g/mL).Under these conditions,the average extraction yield of flavonoids was 57.46 mg/g,and the average extraction yield of polyphenols was 79.81 mg/g.The extract from grape peels and seeds exhibited significant antioxidant activity.
deep eutectic solvent methodresponse surface optimization experimentflavonepolyphenol
维普
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