首页|响应面法优化龙骨风多酚提取工艺及其活性的初步研究

响应面法优化龙骨风多酚提取工艺及其活性的初步研究

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试验以瑶药龙骨风为原材料,通过单因素试验和响应面法对龙骨风多酚提取工艺进行优化,并对其抗氧化活性和抑菌活性进行初步研究.结果表明:超声温度51 ℃、料液比1∶38 g/mL、超声时间52 min是龙骨风多酚超声辅助最优提取工艺,多酚提取量达51.658 mg/g;在试验质量浓度内,随着龙骨风多酚样液浓度的增加,总抗氧化能力升高,但其总抗氧化能力小于VC的总抗氧化能力;龙骨风多酚对大肠杆菌和金黄色葡萄球菌均具有一定的抑菌效果,最低的抑菌浓度为50mg/mL.此研究为龙骨风多酚的开发与利用提供了基础数据.
Optimization of Polyphenol Extraction Process of Alsophila Spinulosa by Response Surface Methodology and Preliminary Study of the Activity
In this experiment,we used Alsophila spinulosa as the test raw material.The single-factor test and response surface optimization of the extraction process of polyphenols were used from A.spinulosa and preliminarily investigating the antioxidant activity and bacteriostatic activity.The results showed that the optimal process for extracting polyphenols from A.spinulosa was a material-liquid ratio of 1∶38 g/mL,ultrasonic time of 52 minutes,and ultrasonic temperature of 51 ℃.The extracted amount of A.spinulosa polyphenols reached 51.658 mg/g;The total antioxidant capacity was elevated with the increase of the concentration of A.spinulosa polyphenol samples within the experimental mass concentration,but its total antioxidant capacity was smaller than that of VC;The antimicrobial effect of A.spinulosa polyphenols on Escherichia coli and Staphylococcus aureus was observed,with the lowest inhibitory concentration being 50 mg/mL.This study provides basic data for the development and utilisation of Alsophila spinulosa polyphenols.

Alsophila spinulosapolyphenolsresponse surface optimizationantioxidantbacteriostatic activity

郭松、卢全、徐停停、崔永存、梁湘兰、张秋丽

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广西科技师范学院(来宾 546199)

龙骨风 多酚 响应面优化 抗氧化 抑菌性

2024

食品工业
上海市食品工业研究所

食品工业

影响因子:0.47
ISSN:1004-471X
年,卷(期):2024.45(11)