Effect of Thick and Thin Cigarette Smoke on Gut Microbiota and Metabolites of in Mice
To explore the effects of thick and thin cigarette smoke on gut microbiota and metabolites in mice,the C57BL/6 male mice were treated with passive smoking through typical thick and thin cigarettes.The changes of intestinal flora and metabolites in mice were detected by 16S rRNA sequencing and ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The association between differential flora and metabolites was analyzed and constructed by Spearman.The results showed that comparing with the control group,passive smoking treatment did not significantly affect the weight and colon length of mice.16S rRNA sequencing showed that the impact on Bacteroidetes and Firmicutes was not significant at the phylum level,but the genus level,cigarette smoke mainly had a significant impact on the Akkermansiaceae,Helicobacteraceae and Lactobacillaceae.Fecal metabolomics results showed that the cigarette smokes treatment significantly affected the Cyanoamino acid metabolism and Tryptophan metabolism,intervened significantly in the generation of twelve differential metabolites.Spearman analysis showed that the strong correlation between theintestinal bacteria and potential biomarkers was also found.This study preliminarily demonstrated that after passive absorption by thick and thin cigarette smoke,the gut microbiota of mice showed partial ecological imbalance and changes in metabolites.
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维普
