Study on the Differences in Polysaccharide and Louis-Ergothioneine in Black Truffle(Tubermeranosporum)from Different Origins
In this paper,the wild black truffle extracts were collected from Anshan City,Liaoning Province,Kunming City,Yunnan Province and Gongshan County,Yunnan Province,which were extracted by heating reflux method and alcohol precipitation method,the multi-component black truffle extract was prepared by freeze-drying method,the polysaccharide content in the black truffle extract under different origins and different treatment methods was studied by phenol-sulfuric acid method,and the liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was established.The content of L-ergothioneine in the extracts of samples from different origins was determined.According to the results of polysaccharide content extracted from water,the order was Anshan(346.40±0.51 mg/g)in Liaoning Province＞Kunming in Yunnan Province(316.52±51.93 mg/g)＞Gongshan in Yunnan Province(236.75±4.59 mg/g).According to the results of black truffle water extraction alcohol precipitate polysaccharides,the order was Gongshan in Yunnan(611.84±4.43 mg/g),＞Kunming in Yunnan(420.31±3.45 mg/g),＞Anshan in Liaoning(351.36±5.32 mg/g).The results of L-ergothioneine content were determined,and the order was Kunming,Yunnan Province(20.62±0.86 ng/g)＞Gongshan County,Yunnan Province(26.03±1.71 ng/g)＞Anshan,Liaoning(19.51±0.44 ng/g).The total polysaccharide content of black truffle was quite different among the wild black truffle samples from different origins,and the ergothioneine content was also significantly different.In this paper,the content of polysaccharides and ergothioneine in black truffle samples from different origins was detected,the gap between the two effective substances in wild black truffle samples from different origins was determined,and the optimal production areas of wild black truffle were screened,which provided a corresponding theoretical basis for the further development and application of black truffle in cosmetics,medicine,food and other fields.
black trufflepolysaccharideLC-MS/MSlouis ergothioneine
万方数据
维普
