Effect of calcium and phosphorus on accumulation of vitamin E in wheat embryo callus
The effect of the content of calcium element and phosphorus element in the culture on accumulated vitamin E of wheat embryo callus was studied.The mathematical models were established to describe growth kinetics and accumulation of vitamin E for wheat embryo callus cells.Based on the most accumulation of secondary metabolites Vitamin E as an object,the best combination of Ca element, P element in medium was selected by test optimum.The results showed that the production of vitamin E in B, medium was most at the CaCl_2·2H_2O 150mg/L and NaH_2PO_4 · H_2O 300mg/L.The fitting degree of kinetic model of Vitamin E accumulation was 97.96％,and the fitting degree of kinetic model of cell growth was 95.57％, nonlinear relation was well.The trace element Ca and P affect vitamin E accumulates in medium for wheat germ callus,the influence of element P was more remarkable than that of element Ca.
wheat embryoaccumulation of vitamin Ecalcium and phosphorusnonlinear models
国家科技期刊平台
NSTL
万方数据
维普
