沙棘叶多糖的提取工艺及抗氧化作用的研究
Study of extraction and antioxidant of polysaccharide from Hippohae rhamnoides L.leave
包怡红 1秦蕾 1王戈2
作者信息
- 1. 东北林业大学林学院,黑龙江哈尔滨,150040
- 2. 中共黑龙江省委办公厅,黑龙江哈尔滨,150007
- 折叠
摘要
以沙棘叶多糖得率为指标,通过单因素及正交实验,对水提、微波辅助及超声波辅助三种提取方法进行比较,超声波辅助法的最佳提取条件为:液固比60:1,超声波功率700W,超声波作用时间40min.在此条件下,超声波法的最佳提取率为7.50%,高于微波法的最佳提取率7.05%和水提法的最佳提取率6.24%.实验结果表明:沙棘叶多糖具有一定的体外抗氧化能力,其中当多糖浓度为1200μg/mL时,对O_2~-·的清除率比同浓度的V_C还高18.92%.
Abstract
Taking the rate of polysaccharide from Hippohae rhamnoides L.eaves as evaluation index, and through single factor and orthogonal tests, the three methods of extraction: water, microwave-assisted and ultrasonic-assisted,were compared.The results showed the best ultrasonic-assisted extraction rate of 7.50%, higher than the microwave method the best rate of 7.05% and water methods the best rate of 6.24%. The best conditions of ultrasonic extract methods were as follows: liquid-solid ratio 60: 1, the ultrasonic power 700W, ultrasonic time 40min.The polysaccharide extracted from Hippohae rhamnoides L.leave had certain degree antioxidant capacities in vitro,for example,when the concentration of polysaccharide was 1200μg/mL,the removal effect towards O_2~-·was of higher than V_C by 18.92% .
关键词
提取/多糖/沙棘叶/体外抗氧化Key words
extraction/polysaccharide/Hippohae rhamnoides L.leave/antioxidant in vitro引用本文复制引用
出版年
2010
国家科技期刊平台
NSTL
维普
万方数据