首页|果糖与氨基酸美拉德反应产物的抗氧化性研究

果糖与氨基酸美拉德反应产物的抗氧化性研究

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为分析果糖参与的美拉德反应对氨基酸、多肽与蛋白质理化性质及抗氧化性的影响,利用果糖与四种代表性氨基酸(赖氨酸、甘氨酸、组氨酸、半胱氨酸)进行模式美拉德反应;考察了反应过程中体系的褐变与pH变化,并测试了体系的ABTS、DPPH自由基清除能力,铁氰化钾还原能力以及铁铜离子螯合能力在美拉德反应前后的变化.结果表明,反应体系的酸度以及褐变程度均随美拉德反应的进行逐渐增加,体系清除自由基能力与还原能力也大幅度提高.此外,氨基酸的结构与种类对美拉德反应的进行以及相应产物的性能都会产生较大影响.
Study on maillard reaction of D-fructose and amino acids and antioxidant activity of their products
To analyze how the Maillard reaction of D-fructose influences the physiochemical properties and the antioxidant activity of amino acid, polypeptide and protein,the four Maillard reaction models were built between D-fructose and amino acids (lysine, glycine, histidine and cysteine, respectively).The pH value and absorbance of the systems during the Maillard reaction were measured.The change of the scavenging effect on free radicals(ABTS and DPPH),reducing power and the metal-ion chelating activities of Maillard reaction products (MRPs)in the systems were also evaluated.The results showed that the browning and acidity of the MRPs kept increasing with the heating time.The radical scavenging activity and reducing power of MRPs were enhanced greatly.In addition,the species and structure of amino acid had an important influence on the proceeding of the Maillard reaction and the properties of corresponding MRPs.

D-fructoseMaillard reactionantioxidant activitybrowning

张晓溪、曾艳、张泽生、孙媛霞

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天津科技大学食品工程与生物技术学院,天津300457

中科院天津工业生物技术研究所,天津300308

果糖 美拉德反应 抗氧化能力 褐变

国家高技术研究发展计划(863计划)天津市自然科学基金

2009AA02Z20110JCYBJC08900

2011

食品工业科技
北京一轻研究院

食品工业科技

CSTPCDCSCD北大核心
影响因子:0.842
ISSN:1002-0306
年,卷(期):2011.32(6)
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